Cloning and Disease Resistance Analysis of the Maize ZmBON3 Gene

(1) Corn is the most widely planted food crop, feed and economic crop in world, plays an important role agricultural production national economy development. The copine gene, also known as BONZAI encodes a Ca2+-dependent phospholipid membrane binding protein that present eukaryotes. It has been found negative regulator of disease resistance regulation key plants’ response. In this study, Agrobacterium-tumefacien-mediated method was used to successfully obtain T2 generation ZmBON3-gene-overexpressing plants gene-edited plants. Related phenotypes molecular identification showed overexpression significantly reduced, increased, which verified ZmBON3 gene regulatory gene. By detecting physiological indexes related defense, it content H2O2 enzyme active water CAT, POD, SOD, PAL ZmBON3-gene-edited higher than those control plants, SOD activity lowest. expression genes defense-related signaling pathways, may be involved defense processes mediated by R SA pathway, JA ABA pathway. addition, ZmBON3-geneedited obvious dwarf phenomenon at seedling stage, but did not affect ear length, axis diameter, row number, grain color.